Journal: Hormones and behavior

Article Title: Nanoparticle encapsulation increases the brain penetrance and duration of action of intranasal oxytocin

doi: 10.1016/j.yhbeh.2018.12.011

Figure Lengend Snippet: Validation studies to determine the integrity of the artificial blood-brain barrier formed by BEND3 cells. TOP: Representative picture of a double setup Ussing chamber for the measurement of transepithelial electrical resistance and macromolecular transport. MIDDLE: Representative photomicrograph of monolayer and tight junction formation by BEND3 cells. BOTTOM: Barrier integrity was confirmed by examining transfer of trypan blue dye across the membrane using spectrophotometry at 540 nm. All points represent the mean ± SEM, and any points without error bars indicate instances in which the SEM is encompassed by the data point. Abscissae: Hours following the introduction of the trypan blue dye to the left compartment of the Ussing chamber. Ordinates: Percentage of the dye that crossed to the right side of the Ussing chamber normalized to the amount remaining on the left side. All assessments were conducted in triplicate. * = p < 0.05 in comparison to the presence of the cellular barrier.

Article Snippet: To study the transport of nanoparticle formulations across the BEND3 BBB model barrier, we used a complete Ussing chamber system with a multichannel voltage-current clamp and the EasyMount Ussing chambers (Model VCC MC8; P2300; Physiologic Instruments Inc.; San Diego, CA).

Techniques: Spectrophotometry

Journal: Hormones and behavior

Article Title: Nanoparticle encapsulation increases the brain penetrance and duration of action of intranasal oxytocin

doi: 10.1016/j.yhbeh.2018.12.011

Figure Lengend Snippet: Transport of fluorescein isothiocyanate (FITC) encapsulated in Tf-coated nanoparticles across the artificial blood-brain barrier formed by BEND3 cells. The extent of transport was assessed by quantifying the fluorescence intensity of FITC using a fluorescent spectrophotometer at an excitation wavelength of 485 nm and an emission wavelength of 528 nm. All points represent the mean ± SEM, and any points without error bars indicate instances in which the SEM is encompassed by the data point. Abscissae: Hours following the introduction of the FITC dye to the left compartment of the Ussing chamber. Ordinates: Percentage of FITC that crossed to the right side of the Ussing chamber normalized to the amount remaining on the left side. All assessments were conducted in triplicate.

Article Snippet: To study the transport of nanoparticle formulations across the BEND3 BBB model barrier, we used a complete Ussing chamber system with a multichannel voltage-current clamp and the EasyMount Ussing chambers (Model VCC MC8; P2300; Physiologic Instruments Inc.; San Diego, CA).

Techniques: Fluorescence, Spectrophotometry